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GE17-0413-01

HiTrap® Blue High Performance

Cytiva 17-0413-01, pack of 1 × 5 mL

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

ligand

Cibacron Blue F3G-A

Conditionnement

pack of 1 × 5 mL

Fabricant/nom de marque

Cytiva 17-0413-01

Paramètres

42 psi

Taille du lit

16 mm × 25 mm

Volume du lit

5 mL

D.I. de la colonne

16 mm

Taille

Taille moy. des particules

34 μm

cleaning

3-13

Plage de travail

4-12

Description générale

HiTrap® Blue HP is prepacked with Blue Sepharose High Performance for capture of a wide range of biomolecules, including: albumin, interferon, a broad range of nucleotide-dependent enzymes, α2-macroglobulin, coagulation factors, and related proteins.

Application

Blue Sepharose High Performance is a group specific adsorbent with affinity for a wide variety of enzymes. The dye ligand, Cibacron Blue F3G-A, is covalently attached to the matrix via the triazine part of the dye molecule. Some Proteins interact biospecifically with the dye due to its structural similarity with nucleotide cofactors, while others, such as albumin and interferon, bind in a less specific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand.

Caractéristiques et avantages

  • Can be used for depletion of albumin from serum.
  • Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked Agarose medium and is stable over a pH range of 4 to 12 for use and 3 to 13 for cleaning.
  • The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC System.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Produit(s) apparenté(s)

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


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Certificats d'analyse (COA)

Lot/Batch Number

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Articles

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocoles

This page shows how to remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from GE Healthcare.

This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.

Performing a Purification of NAD+-dependent dehydrogenases and ATP-dependent kinases with 5’ AMP Sepharose 4B, HiTrap Blue HP and Blue Sepharose 6 Fast Flow

This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from GE Healthcare.

Afficher tout

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