GE17-0404-01

HiTrap^® Protein G High Performance

Cytiva 17-0404-01, pack of 5 × 1 mL

• Synonyme(s) : Protein purification column
• Code UNSPSC : 23151816
• Nomenclature NACRES : NA.56

Propriétés

• ligand: recombinant protein G lacking albumin-binding region
• Conditionnement: pack of 5 × 1 mL
• Fabricant/nom de marque: Cytiva 17-0404-01
• Conditions de stockage: (20% Ehtanol); (20% Ethanol)
• Paramètres: <4 mL/min flow rate; 42 psi
• Taille du lit: 7 mm × 25 mm
• Volume du lit: 1 mL
• D.I. de la colonne: 7 mm
• Taille moy. des particules: 34 μm
• cleaning: 2-9
• working range: 3-9
• Capacité: >1 mg binding capacity (lgG/column); 25 mg binding capacity(human IgG/column)
• Température de stockage: 2-8°C

Description

Description générale

HiTrap^® Protein G HP columns are prepacked with Protein G Sepharose^™ High Performance for fast, easy separations of IgG from different species and subclasses.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Caractéristiques et avantages

• High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
• 34 μm bead size for high performance, high-resolution purifications.
• Binds broad range of different species and subclasses of IgG.
• Simple and proven method giving reproducible results.
• Designed for use with syringe, peristalic pump, or chromatography system.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids