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F8192

Sigma-Aldrich

Sérum de veau fœtal

Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonyme(s) :

FBS, FCS, sérum, sérums

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About This Item

Numéro MDL:
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

Source biologique

bovine fetus

Niveau de qualité

Stérilité

sterile-filtered

Gamme de produits

FBS Classic

Composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

Origine

Australia origin

Technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

Impuretés

≤10 EU/mL endotoxin

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Application

FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Notes préparatoires

Prepared by heating at 56°C for 30 minutes.

This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Remarque sur l'analyse

  • Taux d′endotoxines et d′hémoglobine testés
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Articles

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