F2302

Fractalkine, Chemokine Domain from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

• Synonyme(s) : CX3CL-1, Neurotactin
• Numéro MDL: MFCD01635336
• Code UNSPSC : 51111800
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Pureté: >97% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 0.3-3 μg/mL
• Poids mol.: calculated mol wt ~9.5 kDa
• Conditionnement: pkg of 25 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: O35188
• Température de stockage: −20°C
• Informations sur le gène: mouse ... Cx3cl1(20312)

Description

Description générale

CX3CL1 (chemokine (C-X3-C motif) ligand 1) or fractalkine is a membrane protein, which is the only member of CXC3 family. Its chemokine domain (CKD) anchors it to the cell membrane through its mucin-like stalk. It acts as a ligand for the G-protein coupled receptor CX3CR1, which is found on smooth muscle cells, T-cells, natural killer (NK) cells and monocytes. Fractalkine is expressed on epithelial, endothelial and smooth muscle cells and neurons.

Actions biochimiques/physiologiques

CX3CL1 (chemokine (C-X3-C motif) ligand 1) or fractalkine, along with CX3CR1 is involved in various disorders such as atherosclerosis, multiple sclerosis, neuropathic pain and rheumatic disorders. It facilitates the proliferation and survival of primary human vascular smooth muscle cells in an epidermal growth factor receptor (EGR)-dependent manner. It plays an essential role in cell survival and promotes the survival of cells such as monocytes, T-cells and microglia. In humans, it is thought to be involved in atherogenesis, and thus, might have potential as a target in treatment of cardiovascular disease. In patients with sickle cell disease (SCD), the serum levels of this chemokine is increased, thus implicating it in the pathogenesis of inflammation associated with SCD.

Leukocyte chemoattractant protein and member of the chemokine superfamily possessing a CX₃C motif. Mouse fractalkine is chemotactic for neutrophils and T-lymphocytes but not monocytes.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA containing 1.25 mg bovine serum albumin.

Remarque sur l'analyse

The biological activity is measured by its ability to chemoattract freshly isolated peripheral blood lymphocytes.

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H315 - H318
• Conseils de prudence: P280 - P305 + P351 + P338
• Classification des risques: Eye Dam. 1 - Skin Irrit. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable