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Principaux documents

E7033

Sigma-Aldrich

pFLAG-CMV-2 Expression Vector

shuttle vector for intracellular transient expression of N-terminal Met-FLAG

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About This Item

Code UNSPSC :
12352200

Étiquette/Marqueur

FLAG® tagged

Qualité

for molecular biology
shuttle vector for intracellular transient expression of N-terminal Met-FLAG

Forme

buffered aqueous solution

Localisation du marqueur peptidique

N-terminal

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

The pFLAG-CMV-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG® fusion proteins in mammalian cells.

pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.

The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.

Vector Maps and Sequences

Composants

  • pFLAG-CMV-2 Expression Vector 20 μg (E7398) is supplied as 0.5 mg/ml in 10 mM Tris-HCl pH 8.0 in 1 mM EDTA.
  • pFLAG-CMV-2-BAP Control Plasmid 20 μg (P5100) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Principe

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs.

Informations légales

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids


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Jing Ouyang et al.
Blood, 117(16), 4315-4322 (2011-02-09)
Posttransplant lymphoproliferative disorders (PTLDs) are potentially fatal, EBV-driven B-cell malignancies that develop in immunocompromised solid organ or hematopoietic stem cell recipients. In PTLD, the expression of EBV proteins, including latent membrane protein 1 (LMP1) and LMP2A, viral immune evasion strategies
Li Yang et al.
Cancer research, 67(12), 5587-5593 (2007-06-19)
Evidence indicates that the induction of cyclooxygenase-2 (COX-2) and high prostaglandin E2 (PGE2) levels contribute to the pathogenesis of non-small-cell lung cancer (NSCLC). In addition to overproduction by COX-2, PGE2 concentrations also depend upon the levels of the PGE2 catabolic
Michiko Tanaka et al.
Virology journal, 5, 125-125 (2008-10-23)
The herpes simplex virus 1 (HSV-1) UL7 gene is highly conserved among herpesviridae. Since the construction of recombinant HSV-1 with a mutation in the UL7 gene has not been reported, the involvement of HSV-1 UL7 in viral replication has been
Chun-Hung Lin et al.
The FEBS journal, 274(11), 2946-2956 (2007-05-10)
The mammalian nitrilase (Nit) protein is a member of the nitrilase superfamily whose function remains to be characterized. We now show that the nitrilase family member 2 gene (NIT2) is ubiquitously expressed in multiple tissues and encodes protein mainly distributed
Kohsuke Tsuchiya et al.
Journal of immunology (Baltimore, Md. : 1950), 185(2), 1186-1195 (2010-06-23)
Listeria monocytogenes invades the cytoplasm of macrophages and induces the activation of caspase-1 and the subsequent maturation of IL-1beta and IL-18. Although apoptosis-associated speck-like protein containing a caspase-activating and recruitment domain (ASC), an adaptor protein of nucleotide-binding oligomerization domain (Nod)-like

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