E4781

Anti-eRF3a/GSPT1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-Eukaryotic Release Factor 3a, Anti-G1 to S phase transition 1, Anti-GST1, homolog of yeast
• Numéro MDL: MFCD09265357
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 76.5 kDa
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μL
• Concentration: ~1 mg/mL
• Technique(s): immunoprecipitation (IP): 1-2 μg using HeLa cell lysates; indirect immunofluorescence: 5-10 μg/mL using paraformadehyde-fixed HeLa cells; western blot: 0.5-1 μg/mL using HeLa cell lysates
• Numéro d'accès UniProt: P15170
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GSPT1(2935) ; mouse ... Gspt1(14852) ; rat ... Gspt1(24420)

Description

Description générale

Eukaryotic release factor 3 a (eRF3a) is encoded by the gene G1 to S phase transition 1 (GSPT1) in humans. The eRF3a/GSPT1 exon 1 is a translation termination protein characterized with a tri-nucleotide GGC repeat coding for a polyglycine expansion in the amino-terminal end.

Immunogène

synthetic peptide corresponding to amino acids 190-204 of human eRF3a/GSPT1, conjugated to KLH via a C-terminal added cysteine residue. The sequence is conserved in human, rat, and mouse.

Application

Anti-eRF3a/GSPT1 antibody produced in rabbit has been used in immunoprecipitation, indirect immunofluorescence and western blotting.

Actions biochimiques/physiologiques

Eukaryotic release factor 3 a (eRF3a) is proteolytically processed into an IAP (Inhibitors of Apoptosis Proteins)-binding protein. Processed eRF3a interacts with IAPs and can promote caspase activation, IAP ubiquitination and apoptosis. The IAP binding motif is exposed only after proteolytic cleavage of a 69-residue leader sequence. eRF3a also affects mRNA stability and translation initiation by interacting with Poly(A)-binding protein (PABP) and the surveillance factor Upf1.

Eukaryotic release factor 3a (eRF3a) is a translation termination protein that is encoded by G1 to S phase transition 1 gene (GSPT1) in humans. The eRF3a/GSPT1 exon 1 contains a tri-nucleotide GGC repeat coding for a polyglycine expansion in the N-terminal of the protein. It is associated with protein translation as a regulator of apoptosis signal-regulating kinase 1 (ASK1). GSPT1 interacts with ASK1 and enhances ASK1-induced apoptotic activity by activating caspase-3. It plays a novel role in the regulation of ASK1-mediated apoptosis. GSPT1 is found to be up-regulated in various human cancers and is considered as a potential proto-oncogene.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)