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E1385

Sigma-Aldrich

Ethidium bromide solution

BioReagent, for molecular biology, 500 μg/mL in H2O

Synonyme(s) :

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide

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About This Item

Formule empirique (notation de Hill):
C21H20BrN3
Numéro CAS:
1239-45-8
Poids moléculaire :
394.31
Numéro Beilstein :
3642536
Numéro MDL:
MFCD00011724
Code UNSPSC :
41105322
ID de substance PubChem :
24894408
Nomenclature NACRES :
NA.31

Qualité

for molecular biology

Niveau de qualité

200

Gamme de produits

BioReagent

Concentration

500 μg/mL in H2O

Technique(s)

electrophoresis: suitable

Adéquation

suitable for gel electrophoresis

Chaîne SMILES 

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

Clé InChI

ZMMJGEGLRURXTF-UHFFFAOYSA-N

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Application

Ethidium bromide solution has been used to deplete mtDNA to investigate mitochondrial damage signals in autophagy. It has also been used to detect DNA damage using comet assay.
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Actions biochimiques/physiologiques

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Reconstitution

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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