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Key Documents

DUO87002

Sigma-Aldrich

Duolink® PLA® Probe Mouse IgG Isotype Control MINUS from mouse

suitable for proximity ligation assay

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About This Item

Source biologique

mouse (polyclonal)

Niveau de qualité

200

Forme d'anticorps

affinity purified immunoglobulin (Isotype control antibody)

Gamme de produits

Duolink®

Technique(s)

proximity ligation assay: suitable

Adéquation

suitable for brightfield
suitable for fluorescence

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

An immunoglobulin G (IgG) isotype control antibody with the Duolink PLA MINUS oligonucleotide specific for mouse IgG. This is a convenience product to be used in place of a mouse primary antibody to determine non-specific background signal and optimal antibody dilution. The anti-mouse PLA probe MINUS is not necessary when using this product, but must be used in combination with a PLUS PLA probe.

Application

Duolink® proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

Application Note
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
Isotype control PLA probes are used in lieu of a primary antibody in a Duolink® PLA in situ experiment to determine background signal contributed by non-specific binding of the primary antibody. Isotype control PLA probes can also be used to determine the optimal titer conditions of primary antibody PLA probes.

A PLUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.

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Caractéristiques et avantages

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Informations légales

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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