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Key Documents

DNTP10

Sigma-Aldrich

Deoxynucleotide Set, 10 mM

Individual dNTPs for routine PCR; 0.5 mL each

Synonyme(s) :

10mM dNTP set, DNTP 10 mM solution, dNTP set, dNTPs

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About This Item

Code UNSPSC :
41106305
Nomenclature NACRES :
NA.52

Forme

liquid

Concentration

10 mM

Couleur

colorless

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Deoxynucleoside triphosphates (dNTPs) are substrates for DNA polymerizing enzymes and are the essential building blocks of nucleic acid molecules. It aids in the synthesis of copies of the target DNA by providing each of the four bases (dATP, dGTP, dCTP, dTTP).(2) dNTP is a key component of PCR master mixes as it is necessary for DNA amplification reaction.

Application

Deoxynucleotide Set, 10 mM has been used in:
  • polymerase chain reaction (PCR)
  • nested PCR
  • DNA repair
  • the preparation of DNA-duplex

Conditionnement

0.5 mL each of 10 mM dATP, dCTP, dGTP and dTTP

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

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Nicolas A Gillet et al.
Methods in molecular biology (Clifton, N.J.), 1582, 127-141 (2017-03-31)
We describe here a method to identify the position of retroviral insertion sites and simultaneously to quantify the absolute abundance of each clone, i.e., the number of cells having the provirus inserted at a given place in the host genome.
Genetic (RAPD) diversity in two Armadillidium vulgare populations
Homor P, et al.
Tissue Antigens, 34, 19-22 (2003)
Jiannis Ragoussis et al.
Methods in molecular biology (Clifton, N.J.), 338, 261-280 (2006-08-05)
Recent studies on genome-wide localization of transcription factor (TF) binding to DNA have shown that a large proportion of identified sequences do not contain consensus motifs predicted by databases of transcription factor binding sites, such as TRANSFAC. The main limitation
Suzanne Paz et al.
Molecular and cellular biology, 29(12), 3401-3412 (2009-04-22)
Induction of the antiviral interferon response is initiated upon recognition of viral RNA structures by the RIG-I or Mda-5 DEX(D/H) helicases. A complex signaling cascade then converges at the mitochondrial adapter MAVS, culminating in the activation of the IRF and
Adelina Orellana et al.
PloS one, 13(1), e0189247-e0189247 (2018-01-11)
Thymic stromal lymphopoietin (TSLP) is a cytokine released by human lung epithelium in response to external insult. Considered as a master switch in T helper 2 lymphocyte (Th2) mediated responses, TSLP is believed to play a key role in allergic

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