D2196

DAF-FM solution

5 mM in DMSO, ≥97% (HPLC)

• Synonyme(s) : 4-Amino-5-methylamino- 2′,7′-difluorofluorescein, Diaminofluorescein-FM
• Formule empirique (notation de Hill): C₂₁H₁₄F₂N₂O₅
• Numéro CAS: 254109-20-1
• Poids moléculaire : 412.34
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Pureté: ≥97% (HPLC)
• Forme: DMSO solution
• Conditions de stockage: desiccated
• Couleur: colorless to yellow, including pink
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• InChI: 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
• Clé InChI: DIJCILWNOLHJCG-UHFFFAOYSA-N

Description

Application

DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.

Actions biochimiques/physiologiques

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm; Em: 515 nm; dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.

Caractéristiques et avantages

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable