CS0790
Antioxidant Assay Kit
sufficient for 200 tests
Synonyme(s) :
Oxidative Stress Detection Kit
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
sufficient for 200 tests
Niveau de qualité
Méthode de détection
colorimetric
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
Free radicals or reactive oxygen species (ROS) are produced during biochemical redox reactions as part of normal physiological cell metabolism (protection from infectious organisms) and as a response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and g-radiation. Once formed, ROS attack cellular components causing damage to lipids, proteins, and DNA, which can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders.
In the presence of antioxidants the radical cation is suppressed to an extent dependent on the activity of the antioxidant and the color intensity is decreased proportionally. Trolox, a water-soluble vitamin E analogue, serves as a standard or a control antioxidant.
Application
Antioxidant assay kit provides all of the reagents required for an efficient measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates. It has also been used to measure the radical cation (2,2′-azino-di-[3-ethylbenzthiazoline sulphonate]) (ABTS+) scavenging capacity.
It was tested on A431 and CHO cell lysates; rat brain, liver, and kidney lysates; human plasma, serum, urine, and saliva.
Adéquation
Suitable for the measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates
Principe
The principle of the antioxidant assay is formation of a ferryl myoglobin radical from metmyoglobin and hydrogen peroxide, which oxidizes the ABTS (2,2¢-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) to produce a radical cation, ABTS·+ , a soluble chromogen that is green in color and can be determined spectrophotometrically at 405 nm.
Notes préparatoires
Reagents are to be prepared with ultrapure water.
Reconstitution
Myoglobin Solutions are to be stored at -20C after reconstitution.
Trolox Working Solution is to be stored at -20C after reconstitution.
ABTS Substrate Solution is to be stored at 4C for 2 weeks or at -20C for 1 month.
Trolox Working Solution is to be stored at -20C after reconstitution.
ABTS Substrate Solution is to be stored at 4C for 2 weeks or at -20C for 1 month.
Informations légales
Composants de kit seuls
Réf. du produit
Description
- Assay Buffer 10X 30 mL
- Stop Solution 20 mL
- Hydrogen Peroxide, 3% solution 1 mL
- Myoglobin from horse heart 2 x 1
- (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox®) 2 x 1
- ABTS, 10 mg tablets 4 tab
- Phosphate-Citrate Buffer, pH 5 4 tab
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Effect of sourdough fermentation on stabilisation, and chemical and nutritional characteristics of wheat germ.
Rizzello CG, et al.
Food Chemistry, 119(3), 1079-1089 (2010)
Muhammad Arshad et al.
Plant science : an international journal of experimental plant biology, 258, 122-136 (2017-03-24)
Alfalfa (Medicago sativa) is an important forage crop that is often grown in areas that frequently experience drought and water shortage. MicroRNA156 (miR156) is an emerging tool for improving various traits in plants. We tested the role of miR156d in
Grit Kasper et al.
Stem cells (Dayton, Ohio), 27(6), 1288-1297 (2009-06-06)
Progenitor cells such as mesenchymal stem cells (MSCs) have elicited great hopes for therapeutic augmentation of physiological regeneration processes, e.g., for bone fracture healing. However, regeneration potential decreases with age, which raises questions about the efficiency of autologous approaches in
Dave C Francisco et al.
Free radical biology & medicine, 44(4), 558-569 (2007-11-17)
Oxidatively induced stress and DNA damage have been associated with various human pathophysiological conditions, including cancer and aging. Complex DNA damage such as double-strand breaks (DSBs) and non-DSB bistranded oxidatively induced clustered DNA lesions (OCDL) (two or more DNA lesions
Use of sourdough fermentation and pseudo-cereals and leguminous flours for the making of a functional bread enriched of γ-aminobutyric acid (GABA).
Coda R, et al.
International Journal of Food Microbiology, 137(2), 236-245 (2010)
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique