CN402-05

Canine Chondrocytes: CnC (Cryovial)

• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• Source biologique: canine articular cartilage (normal)
• Conditionnement: pkg of 500,000 cells
• Fabricant/nom de marque: Cell Applications, Inc
• Mode de croissance: Adherent
• Caryotype: 2n = 78
• Morphologie: chondrocyte
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): arthritis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

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Canine Chondrocytes (CnC) are derived from normal canine articular cartilage where they produce and maintain the extracellular matrix of cartilage. Following monolayer culturing, CnC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system. Alginate microspheres provide more physiological conditions for the chondrocytes and allow them to sustain normal proteoglycans production and retention. Because of the prevalence of degenerative joint disease in geriatric dogs, CnC can be a useful tool for veterinary practice.

Canine Chondrocytes (CnC) were utilized to evaluate anti-inflammatory and anti-oxidant activity of Glyco-Flex III (Yáñez, 2008) and trans-pterostilbene (Remsberg, 2008). They were also used in material studies aimed to develop optimal surfaces/scaffolds for engineered tissues, cellular arrays, biosensors and lab-on-chip devices (Wright, 2012; Shaik 2013a,b).

Origine de la lignée cellulaire

Cartilage

Application

production and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regeneration

Composants

Canine Chondryocyte Basal Medium containing 10% FBS & 10% DMSO

Notes préparatoires

• 1st passage, >500,000 cells in Canine Chondryocyte Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 10 doublings

Procédure de repiquage

Please refer to the CnC Culture Protocol.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable