TFT B1

94102701, Reactivity: Anti-Ricin β-chains

• Code UNSPSC : 41106514

Propriétés

• Source biologique: Mouse x Mouse Hybridoma
• Conditionnement: tube of 5 μg 94102701-DNA-5UG; pkg of vial of cells 94102701-1VL
• ImmunoDon: BALB/c mouse spleen
• Myélome: Sp2/5 (clone of SP2/0 Ag-14)
• Réactivité: Anti-Ricin β-chains
• Technique(s): cell culture | mammalian: suitable
• Isotype: IgG1
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse Sp2/5 x Mouse BALB/c spleen immunised with ricin ß chain

Description de la lignée cellulaire

Produces a monoclonal antibody (IgG1) which reacts with the ß chain of the plant toxin ricin.

Immunogène

Ricin ß chain

Application

Has been used in a protocol for purifying the α chains (A1 and A2) of ricin with minimal cross-contamination for the preparation of immunotoxins.

Milieu de culture

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Procédure de repiquage

Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO₂; 37°C.

Autres remarques

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