RPMI 8226

87012702, human blood, Not specified

• Synonyme(s) : RPMI8226 Cells, Roswell Park Memorial Institute 8226 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: RPMI 8226, 87012702
• Source biologique: human blood
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Not specified
• Produits: Immunoglobulin G (Ig) lambda light chain
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human myeloma

Description de la lignée cellulaire

Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.

Application

RPMI 8226 has been used:

• to grow human myeloma cell lines
• to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
• to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.

Autres remarques

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