RAJI′A′

85022102

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human lymph
• Mode de croissance: Suspension
• Caryotype: Modal no. 47-48
• Morphologie: Fibroblast-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Burkitt′s lymphoma

Description de la lignée cellulaire

Clone of RAJI selected for adherence to glass or plastic. Fibroblast like cell line. RAJI ′A′ was developed from the adherent sub-population of RAJI cells. Karyotype analysis demonstrated that RAJI ′A′ contains typical human chromosomes and the modal total chromosome number (47-48) is the same as that of RAJI. RAJI ′A′ is also identical to RAJI with respect to isoenzyme composition, susceptibility to EBV infection and inducibility of latent EBV. Ethnicity: Black.

Application

Tumourigenicity studies

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 17,19

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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