W6/1

13082201, from Mouse x Mouse Hybridoma

• Code UNSPSC : 41106514

Propriétés

• Source biologique: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myélome: P3-NS1/1-Ag4.1
• Réactivité: Human ′A′ erythrocytes
• Technique(s): cell culture | mammalian: suitable
• Isotype: IgM
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description de la lignée cellulaire

The W6/1 hybridoma cell line secretes monoclonal IgM antibodies reactive with a polymorphic determinant of human erythrocytes which correlated with blood group A.

Immunogène

Human tonsil membrane

Application

Human erythrocyte marker.

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Procédure de repiquage

Seed at 5 x 10⁴ cells/ml. Subculture when cells reach 5 x 10⁵ -1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. Start in 20% FBS when recovering from frozen.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.