54SCC3

8062561, Mouse x Rat Hybridoma nerve, oligopolar

• Code UNSPSC : 41106514

Propriétés

• product name: 54SCC3, 08062561
• Source biologique: Mouse x Rat Hybridoma nerve
• Mode de croissance: Adherent
• Caryotype: Modal No. varied with passage see reference
• Morphologie: oligopolar
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat glioma x mouse L cell fibroblast hybrid

Description de la lignée cellulaire

Hybrid generated by fusion of bromodeoxyuridine-resistant mutant of rat glioma cell line C6 (C6-BU-1, Sigma Catalogue number 08062563) and the 6-thioguanine-resistant mouse L cell fibroblast line A9 (Sigma Catalogue number 08062526) using inactivated Sendai virus. Cells formed from such a fusion are multipolar and extend small processes.

Milieu de culture

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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