C6-4-2

8062524, rat brain, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: C6-4-2, 08062524
• Source biologique: rat brain
• Mode de croissance: Adherent
• Caryotype: modal No varied with passage
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat glioma x rat glioma hybrid

Description de la lignée cellulaire

Pseudohexadecaploid cell line generated by fusion of the 8-bromodeoxyuridine resistant mutant rat octaploid glioma cell line C6-3-BU-8 and the 6-thioguanine-resistant mutant rat octaploid glioma cell line C6-3-TG-3. C6-4-2 cells provide a suitable model system for studying the regulation of the membrane potential by peptide hormones.

Application

electrophysiology

Milieu de culture

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C

Autres remarques

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