CHSE-214

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 21714, fish embryo, Fibroblast-like

• Synonyme(s) : CHSE/F
• Code UNSPSC : 41106514

Propriétés

• Source biologique: fish embryo
• Conditionnement: tube of 5 μg 00021714-DNA-5UG; pkg of vial of cells 00021714-1VL
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Common bluegill (Lepomis macrochirus), normal

Description de la lignée cellulaire

The fish line CHSE/F was derived from Common bluegill (Lepomis macrochirus) embryo. It is susceptible to various fish viruses including infectious hematopoietic necrosis virus (IHNV); viral haemorrhagic septicaemia virus (VHSV); spring viraemia of carp virus (SVCV); and infectious pancreatic necrosis virus (IPNV).
ECACC has been advised by the CCLV that this cell line is derived from Lepomis macrochirus (Common bluegill) not Oncorhynchus tshawytscha (Chinook salmon) as originally reported. This has been confirmed by DNA sequencing at ECACC. The CCLV have changed the cell line name to CHSE/F to indicate this. The line is used in viral diagnostics and gives better results than BF-2 (Sigma Catalogue number 00021712), a commonly used cell line of Lepomis macrochirus. The sequence of both lines (BF-2 and CHSE/F possess the same nucleotide exchange but are different in culture behaviour and in virus propagation.

Application

Fish virus studies

Milieu de culture

EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 120mg/l Sodium Pyruvate(NaP) (S8636) + 10% FBS / FCS (F2442).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; grow cells in dark (e.g. wrapped in foil) at 20-26ºC with 26ºC optimum temperature; monolayer is stable for at least 10 days with maintenance medium containing 1-2% FBS.

Autres remarques

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