C9830
Chitosanase from Streptomyces griseus
lyophilized powder, >50 units/mg protein (Bradford)
Synonyme(s) :
Chitosan N-acetylglucosaminohydrolase
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Forme
lyophilized powder
Niveau de qualité
Activité spécifique
>50 units/mg protein (Bradford)
Produit purifié par
chromatography
Solubilité
H2O: soluble, clear to slightly hazy, colorless (10U/0.3mL)
Activité étrangère
chitinase <1.0 units/mg protein
Température de stockage
−20°C
Description générale
Chitosanase is synthesized from Streptomyces griseus as an extracellular secreted enzyme and corresponds to a molecular weight of 34 kDa. It has a temperature and pH optimum of 60°C and 5.7, respectively. Chitosanase belongs to the glycosyl hydrolase family and comprises α-helical tertiary structure similar to lysozyme with substrate-binding cleft placed between two lobes.
Application
Chitosanase from Streptomyces griseus has been used in a study to assess the effect of chitin sources on production of chitinase and chitosanase. Chitosanase from Streptomyces griseus has also been used in a study to investigate the effective production of chitinase and chitosanase by Streptomyces griseus HUT 6037 using colloidal chitin and various degrees of deacetylation of chitosan.
Chitosanase from Streptomyces griseus has been used in the digestion of lacrimal gland (LG) tissues.
The enzyme from Sigma has been used for the hydrolysis of the crude cell wall preparation of oomycete Aphanomyces euteiches, a major parasite of legume plants. It has also been used for the enzymatic hydrolysis of the fully de-N-acetylated chitosan to get chitosan oligomer mixtures during the preparation of biocompatible chitosan-alginate gel.
Actions biochimiques/physiologiques
Chitosanase catalyzes the endohydrolysis of N-acetylated chitosan specifically at the of β(1,4) linkages between N-acetyl-D-glucosamine and D-glucosamine residues. Chitosanase from Streptomyces griseus is capable of hydrolyzing both chitosan and carboxymethyl cellulose. Chitosanase is found in several types of microorganisms and is used for the lysis of fungal cell walls.
Définition de l'unité
One unit will release 1 μmole of glucosamine from chitosan per min at pH 5.0 at 37 °C as measured in the fluorimetric assay of Osswald, et al. (1992).
Forme physique
Lyophilized powder containing potassium phosphate buffer salts
Notes préparatoires
For long term solution storage, reconstitute vial with 50% glycerol solution. Dilute to working concentrations with a 0.1% BSA solution.
Autres remarques
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Les clients ont également consulté
Ilham Badreddine et al.
Eukaryotic cell, 7(11), 1980-1993 (2008-09-23)
Chitin is an essential component of fungal cell walls, where it forms a crystalline scaffold, and chitooligosaccharides derived from it are signaling molecules recognized by the hosts of pathogenic fungi. Oomycetes are cellulosic fungus-like microorganisms which most often lack chitin
Thang Trung Khong et al.
Biomacromolecules, 14(8), 2765-2771 (2013-06-29)
Biocompatible hydrogels are very interesting for applications in, e.g., tissue engineering and for immobilization of cells, such as calcium-alginate gels where the calcium ions form specific interactions with the guluronic acid units. We here report on a new gelling system
Chitosanase from Streptomyces griseus.
A Ohtakara
Methods in enzymology, 161, 505-510 (1988-01-01)
Toshiaki Tanabe et al.
Bioscience, biotechnology, and biochemistry, 67(2), 354-364 (2003-05-06)
Streptomyces griseus HUT 6037 inducibly produced two chitosanases when grown on chitosan. To elucidate the mechanism of degradation of chitinous compound by this strain, chitosanases I and II of S. griseus HUT 6037 were purified and characterized. The purified enzymes
Pascal Viens et al.
Marine drugs, 13(11), 6566-6587 (2015-11-01)
Chitosanases, enzymes that catalyze the endo-hydrolysis of glycolytic links in chitosan, are the subject of numerous studies as biotechnological tools to generate low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS) from native, high molecular weight chitosan. Glycoside hydrolases belonging
Articles
Enzymatic Assay of Chitosanase from Streptomyces Species
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