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Key Documents

C8743

Sigma-Aldrich

Cholesterol Dehydrogenase from Nocardia sp.

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About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
1.1.1.B40
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Produit recombinant

expressed in Streptomyces lividans

Forme

lyophilized solid

Activité spécifique

≥18 unit/mg solid

Poids mol.

~37 kDa by SDS-PAGE

Température de stockage

−20°C

Application

The enzyme sequence is derived from Nocardia sp., and this cholesterol dehydrogenase is expressed in Streptomyces lividans. Cholesterol Dehydrogenase from Nocardia sp. has been used in the preparation of amperometric cholesterol biosensor for cholesterol detection in biological samples and in a study to investigate the atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain with the LDL-receptor tail. Cholesterol dehydrogenase from Nocardia sp. Has also been used in a study to assess organization, dynamics, and segregation of Ras nanoclusters in membrane domains.
Enzyme used in determining cholesterol in serum

Actions biochimiques/physiologiques

Cholesterol dehydrogenase (CDH) is a nicotinamide adenine dinucleotide phosphate (NAD(P)) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Cholesterol detection by using CDH allows direct and easier quantification compared to cholesterol oxidase based assay.
Cholesterol dehydrogenase is a NAD(P) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Molecular mass of the protein is found to be 37 kDa based on SDS-PAGE. Its pH is 4.5.

Définition de l'unité

One unit produces 1.0 μmole of cholesten-4-en-3-one per minute at pH 8.5 at 25 °C.

Forme physique

Supplied as a lyophilized powder

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Lorant Janosi et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(21), 8097-8102 (2012-05-09)
Recent experiments have shown that membrane-bound Ras proteins form transient, nanoscale signaling platforms that play a crucial role in high-fidelity signal transmission. However, a detailed characterization of these dynamic proteolipid substructures by high-resolution experimental techniques remains elusive. Here we use
Takeshi Nomura et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(22), 8770-8775 (2012-05-16)
Mechanosensitive (MS) channels of small (MscS) and large (MscL) conductance are the major players in the protection of bacterial cells against hypoosmotic shock. Although a great deal is known about structure and function of these channels, much less is known
Hay Dvir et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(18), 6916-6921 (2012-04-18)
Hypercholesterolemia, high serum cholesterol in the form of LDL, is a major risk factor for atherosclerosis. LDL is mostly degraded in the liver after its cellular internalization with the LDL receptor (LDLR). This clathrin-mediated endocytosis depends on the protein autosomal

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