C8740
CelLytic™ B Cell Lysis Reagent
For bacterial cell lysis, 10× concentrate
Synonyme(s) :
bacterial cell lysis reagent
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About This Item
Produits recommandés
Forme
liquid
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Application
A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.
TM[="CelLytic"] B Cell Lysis Reagent has been used for lysing E.coli 5-helix bundle protein. It is also suitable for lysing hepatitis B X-interacting protein (HBXIP) and E. coli TG1 cells.
Caractéristiques et avantages
- Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
- Scalable for 1 to 25 grams of bacterial cell paste
- No interference with downstream applications such as affinity chromatography, IP, and Western blotting
- Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents
Autres remarques
10X concentrate of detergent blend contains no buffering components, giving the researcher flexibility to integrate the lysis reagent into their desired system. Also amenable to automation protocols for recombinant protein extraction and purification.
Informations légales
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Skin Irrit. 2
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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