C7611

Monoclonal Anti-Caspase 12 antibody produced in rat

clone 14F7, purified immunoglobulin, buffered aqueous solution

• Synonyme(s) : Anti-CASP-12, Anti-CASP12P1
• Numéro MDL: MFCD03454855
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 14F7, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~50 kDa
• Espèces réactives: rat, mouse
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1-2 μg/mL using whole cell extract of rat kidney cells NRK cells
• Isotype: IgG2a
• Numéro d'accès UniProt: Q920D5
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Casp12(12364) ; rat ... Casp12(156117)

Description

Description générale

Caspase 12 is a protein that belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. The human caspase 12 gene is localized at a single locus within the caspase 1/ICE gene cluster on chromosome 11q22. The functionality is lost in humans and they cannot play a role in Alzheimer′s disease. Caspase-12 is detected in endoplasmic reticulum (ER), plays a role in ER-mediated apoptosis and inflammatory caspase activation pathway.

Monoclonal Anti-Caspase 12 (rat IgG2a isotype) is derived from the 14F7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from CD rats immunized with a recombinant mouse caspase 12. Caspases (cysteine-requiring aspartate protease) are a family of proteases that share similarities in amino acid sequences, structure and substrate specificity. Caspase 12 is ubiquitously expressed in mouse tissues, at high levels in muscle, liver and kidney and at moderate levels in brain, where it is present in cortical neurons, Purkinje cells, brainstem neurons, and olfactory neurons.

Spécificité

Recognizes full length and possibly truncated p20 caspase 12.

Immunogène

recombinant mouse caspase 12 (residues 95-318).

Application

Monoclonal Anti-Caspase 12 has been used in immunoblotting and immunocytochemistry (4% paraformaldehyde-fixed, paraffin-embedded).

Actions biochimiques/physiologiques

Caspase 12 (CASP12) has a downregulatory function during infection and may play a role in protection against inflammatory disease. It is associated with rheumatoid arthritis in African-Americans. Caspase-12 inhibits caspase-1 activation and is associated with susceptibility factor for bacterial sepsis but does not influence the susceptibility to Candida sepsis.

Murine caspase 12 (CASP12) is activated during endoplasmic reticulum stress and activates other caspases which act on substrates, ultimately leading to apoptosis. The protein has been shown to be activated during progression of retinal degeneration in animal models. In mice models, CASP12 has been shown to cause neuronal death resulting from ischemia/reperfusion. Casp12 might stimulate nuclear factor-κB (NF-κB) by directing the post-translational degradation of inhibitor of kappa B (IκBα). Overexpression of caspase 12 induces apoptosis, which is inhibited by the pan caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]- fluoromethyl ketone (z-VAD-FMK).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Notes préparatoires

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable