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C4482

Sigma-Aldrich

CelLytic™ Y Cell Lysis Reagent

For yeast cells

Synonyme(s) :

Yeast cell lysis reagent

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About This Item

Code UNSPSC :
41116134
Nomenclature NACRES :
NA.56

Niveau de qualité

200

Forme

solution

Description générale

CelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.

Application

CelLytic™ Y Cell Lysis Reagent has been used in lysis of yeast cells expressing DNA repair, Rad protein, heat shock protein (ssa1) and human interleukin 4 (hIL4).

Caractéristiques et avantages

  • Fast: Cell lysis in just 20 minutes
  • Efficient: Three times more efficient than glass beads
  • Flexible: Lysis is enhanced by the addition of reducing reagent (DTT)
  • Gentle: Non-denaturing - preserves biological activity

Informations légales

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H319,H412

Classification des risques

Aquatic Chronic 3 - Eye Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

DNA damage induced nucleotide excision repair in Saccharomyces cerevisiae
Singh RK and Krishna M
Molecular and Cellular Biochemistry, 290(1-2), 103-112 (2006)
Expression of recombinant human IL-4 in Pichia pastoris and relationship between its glycosylation and biological activity
Li R, et al.
Protein Expression and Purification, 96, 1-7 (2014)
The beta6beta7 region of the Hsp70 substrate-binding domain mediates heat-shock response and prion propagation
Xu L, et al.
Cellular and Molecular Life Sciences, 75(8), 1445-1459 (2018)

Articles

Yeast Cell Lysis Reagents

Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris and Schizosaccharomyces pombe yeast strains.

Contenu apparenté

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

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