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Key Documents

C-39425

Sigma-Aldrich

Preadipocyte Growth Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

Synonyme(s) :

Recommended with C-27411

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

Forme

aqueous solution

Conditionnement

pkg of 1 vial

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Preadipocyte Growth Medium SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-27411

Application

Preadipocyte Growth Medium is a low-serum (5% V/V) medium developed for the undifferentiated expansion of human preadipocytes. The media are optimized for primary human cells, but can also be used for murine preadipocytes.Recommended for:Human White Preadipocytes (HWP)

Composants

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualité

All lots of PromoCell Preadipocyte/Adipocyte Media are subjected to comprehensive quality control tests using primary human white preadipocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested preadipocytes. In addition, the Preadipocyte Differentiation Medium/Adipocyte Nutrition Medium are tested for the capacity to induce the terminal differentiation of preadipocytes into mature adipocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Notes préparatoires

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlEndothelial Cell Growth Supplement: 0.004 ml / mlEpidermal Growth Factor (recombinant human): 10 ng / mlHydrocortisone: 1 µg / mlHeparin: 90 µg / ml

Stockage et stabilité

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Procédure de repiquage

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

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Articles

Primary Human Preadipocytes and Preadipocyte/Adipocyte Growth & Differentiation Media

Primary human white preadipocytes and culture media for the expansion of undifferentiated preadipocytes, differentiation to adipocytes, and cultivation of mature adipocytes. Protocols for handling, cell passaging, media, and product use.

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