C-39350

Fibroblast Growth Medium 3

SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml

• Synonyme(s) : Recommended with C-23230
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.71

Propriétés

• Forme: liquid
• Conditionnement: pkg of 1 kit
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Fibroblast Growth Medium 3 SupplementPack (basal medium sold separately)


To form a complete medium, it should be combined with the basal medium: C-23230

Application

PromoCell Fibroblast Growth Medium 3 has been developed for the in vitro cultivation of adult Human Cardiac Fibroblasts (HCF). The medium is optimized for primary human cells but can also be used for porcine cardiac fibroblasts.Recommended for:Human Cardiac Fibroblasts (HCF)

Composants

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualité

All lots of PromoCell Fibroblast Growth Medium 3 are subjected to comprehensive quality control tests using primary Human Cardiac Fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested fibroblasts. Approved inhouse lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Notes préparatoires

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.1 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

Stockage et stabilité

Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.Note: The SupplementPack is delivered thawed and can be frozen after arrival without losing any activity.

Procédure de repiquage

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Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids