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Key Documents

COO/COA

C3367

Anti-CIITA antibody, Mouse monoclonal

Name: Anti-CIITA antibody, Mouse monoclonal
Brand: SIGMA

clone 7-1H, purified from hybridoma cell culture

Synonyme(s) :

Anti-C2TA, Anti-MHC Class II Transactivator

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About This Item

Numéro MDL:

MFCD01864254

Code UNSPSC :

12352203

Nomenclature NACRES :

NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

7-1H, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~130 kDa

Espèces réactives

mouse, human

Concentration

~2 mg/mL

Technique(s)

immunocytochemistry: suitable
western blot: 2-4 μg/mL using total cell extract of Raji cells

Isotype

IgG1

Numéro d'accès UniProt

P33076

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CIITA(4261)
mouse ... Ciita(12265)

Description générale

Class II transactivator protein (CIITA) protein contains an acidic region in its N-terminal with transactivation activity, that binds to proteins in the basal transcription machinery and to cAMP response element-binding protein (CREB)-binding protein (CBP). The protein contains motifs for nuclear translocation, GTP binding motif and C-terminal leucine-rich repeats. CIITA is expressed constitutively in B lymphocytes and in immature dendritic cells, while in other cells its expression is induced by IFN-γ .

Monoclonal Anti-CIITA (mouse IgG1 isotype) is derived from the hybridoma 7-1H produced by the fusion of mouse myeloma cells (Sp20 cells) and splenocytes from BALB/c mice immunized with purified N-terminal recombinant CIITA, amino acids 1-350. CIITA is expressed constitutively in B lymphocytes and in immature dendritic cells, while in other cells its expression is induced by IFN-γ.

Monoclonal Anti-CIITA recognizes human1 and mouse † CIITA.

Application

The antibody may be used in immunoblotting (∼130 kDa) and immunocytochemistry.

Actions biochimiques/physiologiques

Class II transactivator protein (CIITA) interacts with specific transcription factors and creates the appropriate transcriptional scaffold required for HLA-D gene expression.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Isoforms of the class II transactivator protein.

Giovanna Barbieri et al.

International immunology, 14(8), 839-848 (2002-07-31)

The class II transactivator (CIITA) controls both the constitutive and IFN-gamma inducible expression of HLA-D genes. In addition, through the squelching of another transactivator CREB-binding protein, CIITA was more recently shown to have a wider cellular function, including cell cycle

CIITA-dependent and -independent class II MHC expression revealed by a dominant negative mutant.

H Zhou et al.

Journal of immunology (Baltimore, Md. : 1950), 158(10), 4741-4749 (1997-05-15)

The MHC class II transactivator gene (CIITA) coordinately controls the expression of the three major human class II genes, HLA-DR, HLA-DQ, and HLA-DP. Indeed, patients with one form of MHC class II immunodeficiency disease, due to defective CIITA genes, lack

Isoforms of the class II transactivator protein

Barbieri G, et al.

International Immunology, 14(8), 839-848 (2002)

Mutation in the class II trans-activator leading to a mild immunodeficiency.

W Wiszniewski et al.

Journal of immunology (Baltimore, Md. : 1950), 167(3), 1787-1794 (2001-07-24)

The expression of MHC class II molecules is essential for all Ag-dependent immune functions and is regulated at the transcriptional level. Four trans-acting proteins control the coordinate expression of MHC class II molecules: class II trans-activator (CIITA), regulatory factor binding

MHC class II-deficient tumor cell lines with a defective expression of the class II transactivator.

Rodrigo Naves et al.

International immunology, 14(5), 481-491 (2002-04-30)

MHC class II expression defects have been evidenced in several human tumor cell lines originating from lung cancers or retinoblastoma. Accordingly, the mouse adenocarcinoma and fibrosarcoma cell lines, RAG and L(tk-), do not express I-A and I-E molecules even when

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