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Merck
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Principaux documents

C-27020

Sigma-Aldrich

Osteoblast Mineralization Medium

Ready-to-use kit including Basal Medium and SupplementMix, 100 ml

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.81

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

wet ice

Description générale

Osteoblast Mineralization Medium (Ready-to-use)

Application

Osteoblast Mineralization Medium has been developed for the efficient induction of strong mineralization in human osteoblast cultures. Extensive extracellular calcium-deposits can be observed after 21 days by following an easy one-step protocol. The medium is optimized for primary human cells, but can also be used for bovine, murine, and rat osteoblasts as well as osteoblast cell lines.Recommended for:Human Osteoblasts (HOB)

Qualité

All lots of PromoCell Osteoblast Mineralization Medium are tested for their capacity to induce mineralization in primary human osteoblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Notes préparatoires

Contains all the growth factors and supplements necessary for mineralization of human osteoblasts.

Stockage et stabilité

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, prewarm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Procédure de repiquage

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Code de la classe de stockage

10 - Combustible liquids

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Certificats d'analyse (COA)

Lot/Batch Number

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Articles

Primary Human Osteoblasts and Osteoblast Growth/Differentiation Media

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

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