C2081

Anti-α-Catenin antibody produced in rabbit

whole antiserum

• Synonyme(s) : Anti-α-Catenin, Alpha Catenin Antibody, Alpha Catenin Antibody - Anti-α-Catenin antibody produced in rabbit
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: whole antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Poids mol.: antigen ~102 kDa
• Contient: 15 mM sodium azide
• Espèces réactives: several mammalian species
• Technique(s): dot blot: suitable using α-catenin peptide amino acids 890-901 conjugated to BSA; immunohistochemistry (frozen sections): 1:2,000 using bovine kidney sections; indirect immunofluorescence: 1:2,000 using cultured MDBK cells; microarray: suitable; western blot: 1:4,000 using cultured MDBK cells extract
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CTNNA1(1495) ; mouse ... Ctnna1(12385) ; rat ... Ctnna1(307505)

Description

Description générale

Catenin are distinct peripheral cytosolic proteins, α, β, and γ -catenin (102 kDa, 94 kDa and 86 kDa, respectively) are found in varying abundance in many developing and adult tissues. Within its conserved regions, α-catenin shows 30% identity to vinculin, a protein found mainly in focal cell-cell and cell substrate adhesions.

Spécificité

Does not cross-react with β-catenin or γ-catenin (plakoglobin).

Immunogène

synthetic peptide corresponding to the C-terminal region (amino acids 890-901) of human/mouse α-catenin conjugated to KLH.

Application

Anti-α-Catenin antibody has been used:

• in dot blot immunoassay
• in immunoblotting
• in immunofluorescence
• in western blotting
• in immunoprecipitation
• in immunofluorescence staining
• in coimmunoprecipitation

Actions biochimiques/physiologiques

Catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Cadherins are transmembrane cell surface glycoprotein molecules that mediate calcium-dependent intercellular interactions and are important for tissue morphogenesis. Catenins link E-cadherin to other integral membrane proteins such as Na⁺ /K⁺ -ATPase, or to cytoplasmic proteins such as fodrin, ankyrin, sarcoma (Src) and Yes kinases and are modulated by Wnt-1 protooncogene. They are considered good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. α-Catenin appears to be capable of interacting with N-cadherin and P-cadherin. Absence of α-catenin is found in certain tumor cell lines. Frequent reduction of α-catenin levels in human carcinomas of the esophagus, stomach and colon is also reported. Prostate cancer development appears to be correlated with α-catenin gene deletions.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable