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APOAC

Sigma-Aldrich

Annexin V-Cy3™ Apoptosis Detection Kit

Synonyme(s) :

Red fluorescent Annexin V apoptosis assay

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32

Utilisation

sufficient for 200 tests

Niveau de qualité

200

Conditionnement

pkg of 1 kit

Technique(s)

flow cytometry: suitable

Application(s)

cell analysis
detection

Méthode de détection

fluorometric

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Caractéristiques et avantages

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Principe

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Informations légales

Cy3 is a trademark of Cytiva

Composants de kit seuls

Réf. du produit
Description
  • Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • C50416-Carboxyfluorescein diacetate 10 mgFDS

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
068M4132V
11180534
125M4193V
11150807
015M4104V

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Anvarbatcha Riyasdeen et al.
Evidence-based complementary and alternative medicine : eCAM, 2012, 136527-136527 (2011-11-25)
Rasagenthi Mezhugu (RGM) is a herbomineral formulation in the Siddha system of traditional medicine and is prescribed in the southern parts of India as a remedy for all kinds of cancers. However, scientific evidence for its therapeutic efficacy in cervical
Christo Paul Preethy et al.
Journal of pharmacology & pharmacotherapeutics, 3(1), 26-34 (2012-03-01)
To find the efficacy of serial extracts of Anisomeles malabarica in inhibiting proliferation of and inducing apoptosis in human cervical cancer cells, SiHa and ME 180, that are HPV 16-positive. The whole plant was extracted in n-hexane, chloroform, ethyl acetate
Phanikrishna Parcha et al.
Chemical biology & drug design, 90(4), 596-608 (2017-03-25)
Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder of the hematopoietic stem cells, characterized at the molecular level by the bcr/abl gene rearrangement. Even though targeting the fusion gene product Bcr-Abl protein is a successful strategy, development of drug
Barbara Sorce et al.
Nature communications, 6, 8872-8872 (2015-11-26)
Little is known about how mitotic cells round against epithelial confinement. Here, we engineer micropillar arrays that subject cells to lateral mechanical confinement similar to that experienced in epithelia. If generating sufficient force to deform the pillars, rounding epithelial (MDCK)
Rajeev Kumar Pandey et al.
PloS one, 6(2), e17093-e17093 (2011-02-25)
Mycobacterium indicus pranii (MIP), also known as Mw, is a saprophytic, non-pathogenic strain of Mycobacterium and is commercially available as a heat-killed vaccine for leprosy and recently tuberculosis (TB) as part of MDT. In this study we provide evidence that
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Articles

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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