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A9919

Sigma-Aldrich

Conjugué anticorps anti-IgG de lapin (molécule entière)–phosphatase alcaline antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

rabbit

Technique(s)

direct ELISA: 1:45,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: 1:100,000-1:200,000 using detecting actin in total cell extract of HeLa cells (5-10 μg per well)

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Binds all rabbit Igs.
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family. It is widely expressed and consists of a gamma (γ) heavy chain in the constant (C) region. IgG is a monomer with a molecular weight of about 150 kDa. The structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa respectively. The primary structure of the antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The Fc fragment contains the CH2 and CH3 domains of the antibody molecule.

Immunogène

purified rabbit IgG

Application

La citrullination de l'antithrombine par PADI4 a été analysée par ELISA à l'aide d'anticorps de chèvre anti-IgG de lapin conjugué à la phosphatase alcaline comme anticorps secondaire, dilué au 1/5 000e dans un tampon carbonate-bicarbonate 0,05 M (pH 9,6).
Goat Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody has been used for immunohistochemistry and western blot applications. The antibody can also be used for direct ELISA at 1:45,000.
Lysates of human fibroblasts and Hela cells were analyzed by western blot using alkaline phosphatase-conjugated goat anti-rabbit IgG as the secondary antibody at a 1:30000 dilution.

Autres remarques

Antibody adsorbed with human IgG.

Forme physique

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide as preservative

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Alex D Greenwood et al.
Molecular neurodegeneration, 6(1), 44-44 (2011-06-28)
Prion diseases such as bovine spongiform encephalopathies (BSE) are transmissible neurodegenerative diseases which are presumably caused by an infectious conformational isoform of the cellular prion protein. Previous work has provided evidence that in murine prion disease the endogenous retrovirus (ERV)
Mari Yotsu-Yamashita et al.
Marine drugs, 16(7) (2018-07-04)
Pufferfish saxitoxin and tetrodotoxin (TTX) binding protein (PSTBP) is a glycoprotein that we previously isolated from the blood plasma of the pufferfish Takifugu pardalis; this protein was also detected in seven species of the genus Takifugu. We proposed that PSTBP
D P Gately et al.
Molecular biology of the cell, 9(9), 2361-2374 (1998-09-03)
Ataxia telangiectasia-mutated gene (ATM) is a 350-kDa protein whose function is defective in the autosomal recessive disorder ataxia telangiectasia (AT). Affinity-purified polyclonal antibodies were used to characterize ATM. Steady-state levels of ATM protein varied from undetectable in most AT cell
The structure of a typical antibody molecule
Immunobiology: The Immune System in Health and Disease (2001)
Silvia Heiss et al.
Microbial cell factories, 15, 50-50 (2016-03-12)
Engineering lactic acid bacteria (LAB) is of growing importance for food and feed industry as well as for in vivo vaccination or the production of recombinant proteins in food grade organisms. Often, expression of a transgene is only desired at

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