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A9594

Sigma-Aldrich

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

Synonyme(s) :

Anti-ddddk, Anti-dykddddk

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About This Item

Code UNSPSC :
41106514
Nomenclature NACRES :
NA.32

Source biologique

mouse

Conjugué

CY3 conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

M2, monoclonal

Forme

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

Espèces réactives

all

Concentration

~1 mg/mL

Technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

Isotype

IgG1

Séquence immunogène

DYKDDDDK

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.

Learn more product details in our FLAG® application portal.

Forme physique

Solution in phosphate buffered saline plus 1% BSA and preservative

Notes préparatoires

Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.

Autres remarques

Suggested concentration of 1-10 mg/ml for immunocytochemistry.

Informations légales

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Saifeng Wang et al.
Genes & development, 33(15-16), 1048-1068 (2019-06-22)
Fetal hematopoietic stem and progenitor cells (HSPCs) hold promise to cure a wide array of hematological diseases, and we previously found a role for the RNA-binding protein (RBP) Lin28b in respecifying adult HSPCs to resemble their fetal counterparts. Here we
Oliver Wicht et al.
Journal of virology, 88(9), 4943-4952 (2014-02-21)
Enveloped viruses carry highly specialized glycoproteins that catalyze membrane fusion under strict spatial and temporal control. To prevent premature activation after biosynthesis, viral class I fusion proteins adopt a locked conformation and require proteolytic cleavage to render them fusion-ready. This
Takeshi Yoshizumi et al.
Biomacromolecules, 19(5), 1582-1591 (2018-03-31)
Selective gene delivery into organellar genomes (mitochondrial and plastid genomes) has been limited because of a lack of appropriate platform technology, even though these organelles are essential for metabolite and energy production. Techniques for selective organellar modification are needed to
Ruizhi Feng et al.
Journal of medical genetics, 53(10), 662-671 (2016-06-09)
TUBB8 is a primate-specific β-tubulin isotype whose expression is confined to oocytes and the early embryo. We previously found that mutations in TUBB8 caused oocyte maturation arrest. The objective was to describe newly discovered mutations in TUBB8 and to characterise
Kunio Yokoyama et al.
The Journal of biological chemistry, 286(7), 5375-5382 (2010-12-21)
Rab27a, a Rab family small GTPase, is involved in the exocytosis of secretory granules in melanocytes and cytotoxic T-cells. Rab27a mutations cause type 2 Griscelli syndrome, which is characterized by immunodeficiency, including uncontrolled macrophage activation known as hemophagocytic syndrome. However
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