A7827
Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse
clone AP1B9, purified immunoglobulin, buffered aqueous solution
Synonyme(s) :
APAAP
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46
Produits recommandés
Source biologique
mouse
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
AP1B9, monoclonal
Description
Concentrate
Forme
buffered aqueous solution
Technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil
Isotype
IgG1
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Description générale
A soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase. The product is a soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase.
Monoclonal Anti-Alkaline Phosphatase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Application
Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse has been used in:
- Immunohistological analysis
- Immunohistochemistry assay
- Immunocytochemical staining
Apoptosis of β-cells in mouse pancreatic sections was analyzed by incubating section with TUNEL and anti-insulin antibody. APAAP was used as the secondary antibody and BCIP/NBT (Sigma) as the substrate.
Actions biochimiques/physiologiques
Mouse Monoclonal Alkaline Phosphatase Anti-Alkaline Phosphatase is a soluble complex formed from levamisole-resistant calf intestinal alkaline phosphatase and purified mouse monoclonal antibody to alkaline phosphatase. The use of a mouse monoclonal alkaline phosphatase anti-alkaline phosphatase (APAAP) complex, in conjunction with a primary mouse antibody and a secondary bridging antibody (anti-mouse IgG), results in an intense signal with a very low background, while avoiding problems inherent to the covalent conjugation of antibodies. The technology of unlabelled antibody peroxidase-anti peroxidase (PAP) and the alkaline phosphatase anti- alkaline phosphatase (APAAP) method has applications in immunostaining. APAAP monoclonal antibodies complex are homogenous and this method is effective and its sensitive.
Forme physique
Solution in 0.05 M Tris buffered saline, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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The onset of p53-dependent apoptosis plays a role in terminal differentiation of human normoblasts
Peller S, et al.
Oncogene, 22(30), 4648-4648 (2003)
Montserrat Biarnés et al.
Diabetes, 51(1), 66-72 (2002-01-05)
We studied the effects of hyperglycemia on beta-cell death and mass in syngeneically transplanted islets. Six groups of STZ-induced diabetic C57BL/6 mice were transplanted with 100 syngeneic islets, an insufficient beta-cell mass to restore normoglycemia. Groups 1, 2, and 3
High-dose chemotherapy and CD34-selected peripheral blood progenitor cell transplantation for patients with breast cancer metastatic to bone and/or bone marrow
Klein JL, et al.
Bone Marrow Transplantation, 28(11), 1023-1023 (2001)
Tumor control in a model of bone marrow transplantation and acute liver-infiltrating B-cell lymphoma: an unpredicted novel function of cytomegalovirus
Erlach KC, et al.
Journal of Virology, 76(6), 2857-2870 (2002)
APAAP complex: production and usage in immunocytochemical and immunohistochemical staining
Naseri M, et al.
Human Antibodies, 16(3-4), 107-115 (2007)
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