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A7420

Sigma-Aldrich

Amyloglucosidase from Aspergillus niger

greener alternative

lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose

Synonyme(s) :

1,4-α-D-Glucane glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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About This Item

Numéro CAS:
9032-08-0
Numéro de classification (Commission des enzymes):
3.2.1.3 (BRENDA, IUBMB)
Numéro CE :
232-877-2
Numéro MDL:
MFCD00081350
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Niveau de qualité

200

Activité spécifique

30-60 units/mg protein (biuret)

Composition

protein, ≥80%

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Impuretés

≤0.02% glucose

Autre catégorie plus écologique

, Enabling

Température de stockage

−20°C

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Catégories apparentées

Description générale

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .

Actions biochimiques/physiologiques

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.

Définition de l'unité

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

Forme physique

Lyophilized powder containing less than 0.02% glucose

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Inhibiteur

Réf. du produit
Description
Tarif

D9305

1-Deoxynojirimycin hydrochloride

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Joto Yoshimoto et al.
Nutrients, 12(6) (2020-06-25)
Legumes are low-carbohydrate food and are abundant in dietary fiber. In order to provide a functional staple food that does not cause a rapid increase in postprandial blood glucose levels, four kinds of legumes were focused on as ingredients. Noodles
Jaetaek Kim et al.
Molecular endocrinology (Baltimore, Md.), 22(11), 2531-2543 (2008-09-20)
The receptors for IGF-I (IGF-IR) and insulin (IR) have been implicated in physiological cardiac growth, but it is unknown whether IGF-IR or IR signaling are critically required. We generated mice with cardiomyocyte-specific knockout of IGF-IR (CIGF1RKO) and compared them with
Brian C Jones et al.
Nature communications, 7, 13856-13856 (2016-12-22)
In gonadal tissues, the Piwi-interacting (piRNA) pathway preserves genomic integrity by employing 23-29 nucleotide (nt) small RNAs complexed with argonaute proteins to suppress parasitic mobile sequences of DNA called transposable elements (TEs). Although recent evidence suggests that the piRNA pathway
Purification and properties of a thermophilic amyloglucosidase from Aspergillus niger
Fogarty WM and Benson CP
Applied Microbiology and Biotechnology, 18(5), 271-278 (1983)
Lizanne Janssens et al.
Aquatic toxicology (Amsterdam, Netherlands), 193, 210-216 (2017-11-04)
Pesticides are causing strong decreases in aquatic biodiversity at concentrations assumed safe by legislation. One reason for the failing risk assessment may be strong differences in the toxicity of the active ingredient of pesticides and their commercial formulations. Sublethal effects
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Protocoles

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

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