A4325
Monoclonal Anti-α-Adaptin antibody produced in mouse
clone 100/2, ascites fluid
Synonyme(s) :
Anti-AP-2
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About This Item
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
100/2, monoclonal
Contient
15 mM sodium azide
Espèces réactives
bovine, human, rat
Technique(s)
western blot: 1:200 using a crude preparation of synaptic vesicles obtained from rat cerebral cortex
Isotype
IgG2a
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... AP2A1(160)
rat ... Ap2a1(308578)
Description générale
Clathrin-coated vesicles facilitate the selective internalization of membrane receptors for lysosomal enzymes from the trans-Golgi network to a pre-lysosomal compartment. Besides clathrin, coated vesicle populations contain the adaptor complexes AP-1 and AP-2, also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor or Assemble Protein 1 (AP1) and Assemble Protein 2 (AP2), respectively. These adaptor proteins mediate the interaction between clathrin and membrane components.
Monoclonal Anti-a-Adaptin (AP-2) (mouse IgG2a isotype) is derived from the 100/2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Adaptor related protein complex 2 subunit alpha 1 (AP2A1) codes for αA adaptin of the AP-2 complex. It is usually seen in clathrin coated vesicles.
The antibody reacts with polypeptides of ~100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from rat pheochromocytoma cultured cells (PC12), neuroblastoma, or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants that occur only in some cell types of brain. The antibody stains the intact α-adaptor, as well as the 37 and 40 kDa fragments, but not the 63-66 kDa group of α-fragments obtained by trypsin cleavage of the α-subunit molecules.
Immunogène
AP-2 adaptor polypeptides from bovine brain.
Application
Immunofluorescence of MDCK cells fixed in methanol was performed using anti-AP1 at a dilution of 1:250 and also western blot at a dilution of 1:1000.
Monoclonal Anti-α-Adaptin antibody produced in mouse has been used in immunoblotting.
Mouse monoclonal anti-α-adaptin antibody has been used:
- as a positive control in immunoblotting assays
- as a primary antibody to detect AP2 levels in tetracycline-repressible stable cell lines
Actions biochimiques/physiologiques
Adaptor related protein complex 2 subunit α 1 (AP2α1) is very essential for epidermal growth factor receptor (EGFR) nuclear translocation, induced by ultraviolet (UV).
Forme physique
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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R A Warren et al.
The Journal of biological chemistry, 273(27), 17056-17063 (1998-06-27)
Endocytosis of surface proteins through clathrin-coated pits requires an internalization signal in the cytoplasmic domain. Two types of internalization signal have been described: one requiring a tyrosine as the critical residue (tyrosine-based motif), and the other consisting of either two
Molecular cloning of novel transcripts of the adaptor-related protein complex 2 alpha 1 subunit (AP2A1) gene, using Next-Generation Sequencing
Adamopoulos PG, et al.
Gene, 678, 55-64 (2018)
Rajesh Abraham Jacob et al.
Virology, 509, 1-10 (2017-06-04)
Acquired Immune Deficiency Syndrome is characterized by a decline in CD4
H C van Leeuwen et al.
Journal of cell science, 114(Pt 11), 2115-2123 (2001-08-09)
p32/gC1qR is a small acidic protein that has been reported to have a broad range of distinct functions and to associate with a wide array of cellular, viral and bacterial proteins. It has been found in each of the main
M S Robinson
Trends in cell biology, 2(10), 293-297 (1992-10-01)
Clathrin has long been known to provide the structural basis for vesicle budding from the plasma membrane during endocytosis, but how is clathrin targeted specifically to some cellular membranes and not others? The answer seems to lie in the adaptors--protein
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