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A4213

Sigma-Aldrich

Monoclonal Anti-Maltose Binding Protein−Peroxidase antibody produced in mouse

clone MBP-17, purified immunoglobulin, lyophilized powder

Synonyme(s) :

Monoclonal Anti-Maltose Binding Protein, Anti-MBP

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About This Item

Code UNSPSC :
12352203

Source biologique

mouse

Niveau de qualité

200

Conjugué

peroxidase conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

MBP-17, monoclonal

Forme

lyophilized powder

Conditionnement

vial of 0.5 mL

Technique(s)

western blot: 1:1,000 using purified, recombinant MBP

Isotype

IgG1

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the MBP-17 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified recombinant MBP fusion protein. Maltose Binding Protein (MBP) is a periplasmic protein, which is specific to bacteria. It has maltodextrin binding site and binds to linear and cyclic maltodextrins. The mature MBP is produced from the pre-protein containing 26 amino acids, as signal peptide.
The antibody recognizes native as well as denatured-reduced forms of purified MBP and MBP fusion proteins.

Immunogène

Purified, recombinant MBP fusion protein

Application

Endogenous intracellular levels of MBP in salmonella cells were determined by western blot analysis using a monoclonal anti-MBP antibody.
Monoclonal Anti-Maltose Binding Protein Peroxidase antibody produced in mouse has been used in:
  • immunoblotting
  • dot blot
  • enzyme-linked immunosorbent assay (ELISA)

Actions biochimiques/physiologiques

Maltose Binding Protein (MBP) is often used to tag recombinant proteins to enhance the yield and facilitate the purification of the fusion product. Additionally, MBP tags can also improve the immunogenicity of the tagged protein. Thus, MBP can also be used as a carrier protein for vaccinations.
Maltose Binding Protein (MBP) mediates uptake of maltose. MBP is a substrate for chaperone secB. It has been reported that the addition of a maltose binding protein (MBP) tag creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product.

Forme physique

Lyophilized in 0.01 M sodium phosphate buffered saline containing 1% bovine serum albumin and 0.05% MIT.

Notes préparatoires

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Reconstitution

The antibody conjugate should be reconstituted with 0.5 mL deionized water.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

P Bellot et al.
Veterinary immunology and immunopathology, 152(1-2), 101-108 (2012-10-20)
Maltose binding protein (MBP) is often fused to a relevant protein to improve its yield and facilitate its purification, but MBP can also enhance the immunogenicity of the fused proteins. Recent data suggest that MBP may potentiate antigen-presenting functions in
Effect of signal peptide on the stability and folding kinetics of maltose binding protein.
Beena K, et al.
Biochemistry, 43(12), 3608-3619 (2004)
The 2.3-A resolution structure of the maltose-or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis.
Spurlino JC, et al.
The Journal of Biological Chemistry, 266(8), 5202-5219 (1991)

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