A1293

Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Goat Anti-Mouse IgG (Fab specific)–AP
• Numéro MDL: MFCD00162620
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.46

Propriétés

• Source biologique: goat
• Produit recombinant: expressed in goat
• Conjugué: alkaline phosphatase conjugate
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: secondary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Espèces réactives: mouse
• Ne doit pas réagir avec: human
• Technique(s): direct ELISA: 1:40,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50; western blot (chemiluminescent): 1:30,000
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice .

Spécificité

Binds all mouse Igs. Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fab fragment. The product does not react with mouse IgG, Fc fragment, or human IgG.

Immunogène

Purified mouse IgG Fab fragment

Application

Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.

Anti-Mouse IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat has been used in:

• enzyme-linked immunosorbent assay (ELISA){47
• immunohistochemistry
• western blot

Actions biochimiques/physiologiques

Immunoglobulin G (IgG) helps in opsonization and antibody dependent cell mediated cytotoxicity. It mainly helps in immune defense.

Autres remarques

Antibody adsorbed with human IgG.

Forme physique

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide

Notes préparatoires

Adsorbed to reduce background staining with human samples.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable