65497

TRIS acetate-EDTA Buffer solution

for Northern and Southern blotting, solution

• Code UNSPSC : 12161700

Propriétés

• Qualité: for molecular biology
• Gamme de produits: BioReagent
• Forme: solution
• Impuretés: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH: 8.3±0.2
• Densité: 1.00 g/mL at 20 °C
• Température de stockage: room temp

Description

Description générale

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This concentrate can be easily diluted to 0.5x before use (with molecular biology grade water).

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. Especially useful for dsDNA separations.

Composants

The TAE liquid buffer is a 1x solution that contains 0.04M TRIS acetate and 0.001M EDTA in water.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves