3011
Transcreener® ADP2 TR-FRET Red Assay
Synonyme(s) :
Transcreener assay
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About This Item
Code UNSPSC :
41122100
Nomenclature NACRES :
NA.84
Produits recommandés
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
The Transcreener® ADP2 TR-FRET Red Assay is a competitive immunoassay for ADP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. Because it is highly selective for ADP, the assay can be used with any enzyme that converts ATP to ADP, regardless of what other substrates are used. Examples of enzymes include protein, lipid, and carbohydrate kinases, ATPases, DNA helicases, carboxylases, and glutamine synthetase.
The Transcreener® assay is designed specifically for high-throughput screening (HTS), with a single addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families.
The Transcreener® ADP2 TR-FRET Red Assay provides the following benefits:
View full Transcreener® product list
The Transcreener® assay is designed specifically for high-throughput screening (HTS), with a single addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families.
The Transcreener® ADP2 TR-FRET Red Assay provides the following benefits:
- Accommodates ATP concentrations ranging from 0.1 μM to 1,000 μM. • Excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10% or less).
- Overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by using a single set of assay reagents that detect an invariant product.
- Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.
- Far-red tracer further minimizes interference from fluorescent compounds and light scattering.
View full Transcreener® product list
Quantité
3011-1K = 1,000 assay, 384-well
3011-10K = 10,000 assay, 384-well
3011-10K = 10,000 assay, 384-well
Forme physique
Kit with buffered aqueous solutions
Informations légales
Transcreener is a registered trademark of BellBrook Labs
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D N Angelov et al.
Developmental neuroscience, 20(1), 42-51 (1998-05-26)
Embryonic stem (ES) cells of the permanent line BLC6 derived from a 129/Sv Gat mouse blastocyst were differentiated as spheroid aggregates (embryoid bodies, EBs) in the presence of retionic acid. After 2 days in suspension, EBs were plated on gelatine-coated
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