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Principaux documents

200P-05N

Sigma-Aldrich

Human Umbilical Vein Endothelial Cells: HUVEC, neonatal, pooled

Synonyme(s) :

HUVEC cells

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About This Item

Code UNSPSC :
41106514
Nomenclature NACRES :
NA.81

Source biologique

human umbilicus (normal veins)

Niveau de qualité

100

Conditionnement

pkg of 500,000 cells

Fabricant/nom de marque

Cell Applications, Inc

Mode de croissance

Adherent

Caryotype

2n = 46

Morphologie

Endothelial

Technique(s)

cell culture | mammalian: suitable

Maladie(s) pertinente(s)

arthritis; diabetes; cancer; cardiovascular diseases

Conditions d'expédition

dry ice

Température de stockage

−196°C

Catégories apparentées

Description générale

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Human Umbilical Vein Endothelial Cells (HUVEC) provide a classic model system to study many aspects of endothelial function and disease, such as normal, abnormal and tumor-associated angiogenesis, oxidative stress, hypoxia and inflammation related pathways in endothelia under normal and pathological conditions, cardiovascular-related complications associated with various diseases, mode of action and cardiovascular protection effects of various compounds, etc.

Origine de la lignée cellulaire

Vein

Application

angiogenesis, cell signaling pathways, migration, invasion, growth factors, tube formation, vascular homeostasis, modulation of blood vessel tone, regulate cell grwoth and ECM deposition, vasculoregulation

Composants

Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO

Notes préparatoires

  • Primary culture, >500,000 cells in Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

Procédure de repiquage

Please refer to the HUVEC Culture Protocol.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Justyna Hajtuch et al.
Frontiers in pharmacology, 12, 733743-733743 (2022-02-15)
Purpose: To study and compare the antibacterial properties and the potential cytotoxic effects of commercially available uncoated silver nanoparticles (AgNPs) with lipoic acid coated silver nanoparticles (AgNPsLA) developed by our group. The antibacterial, cytotoxic, and hemolytic properties of those NPs
Marie-Pierre Hardy et al.
Scientific reports, 9(1), 7203-7203 (2019-05-12)
Endothelial cells have multifaceted interactions with the immune system, both as initiators and targets of immune responses. In vivo, apoptotic endothelial cells release two types of extracellular vesicles upon caspase-3 activation: apoptotic bodies and exosome-like nanovesicles (ApoExos). Only ApoExos are
Concepción Santiago-Fernandez et al.
Frontiers in nutrition, 9, 867745-867745 (2022-05-03)
Little is known about the effect of extra virgin olive (EVOO) and sunflower oil (SO) on the composition of extracellular vesicles (EVs) secreted by endothelial cells and the effects of these EVs on smooth muscle cells (SMCs). These cells play
Sara Dutton Sackett et al.
Scientific reports, 8(1), 10452-10452 (2018-07-12)
Extracellular matrix (ECM) plays an important developmental role by regulating cell behaviour through structural and biochemical stimulation. Tissue-specific ECM, attained through decellularization, has been proposed in several strategies for tissue and organ replacement. Decellularization of animal pancreata has been reported
Wenqing Zhou et al.
Journal of cell science, 133(17) (2020-08-14)
Neutrophils rely on glycolysis for energy production. How mitochondria regulate neutrophil function is not fully understood. Here, we report that mitochondrial outer membrane protein Mitofusin 2 (MFN2) regulates neutrophil homeostasis and chemotaxis in vivo Mfn2-deficient neutrophils are released from the

Protocoles

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

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