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Key Documents

Y1500

Sigma-Aldrich

YPD Agar

Synonyme(s) :

Yeast Extract Peptone Dextrose Agar, YEPD Agar

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About This Item

Code UNSPSC :
50405901
eCl@ss :
42040102
Nomenclature NACRES :
NA.75

Forme

powder

Niveau de qualité

Composition

Agar, 15 g/L
Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L

Application(s)

food and beverages

Adéquation

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

Description générale

YPD agar is a complete yeast culture medium for most yeast species, especially for Saccharomyces cerevisiae. It is also known as YEPD and comprises of yeast extract, bacteriological peptone, glucose, and agar. The components of this media provide all the necessary nutrients for yeast growth. The essential vitamins, minerals and amino acids in the medium acts carbon and nitrogen source.

Application

YPD agar has been used as a culture media:
  • to cultivate S. cerevisiae strains to find a long-term preservation method for a deep space biosensor mission
  • for yeast strains in in vitro broth microdilution (BMD) based on minimum inhibitory concentration (MIC)
  • for Zymoseptori triciti strains for virulence studies

Notes préparatoires

1. Suspend 65 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Ingrid A Edwards et al.
ACS infectious diseases, 3(12), 917-926 (2017-09-30)
Tachyplesin-1 (TP1; 1) is a cationic β-hairpin antimicrobial peptide with a membranolytic mechanism of action. While it possesses broad-spectrum, potent antimicrobial activity, 1 is highly hemolytic against mammalian erythrocytes, which precludes it from further development. In this study, we report
Xiao Liu et al.
Materials (Basel, Switzerland), 13(16) (2020-08-14)
Ammonia nitrogen and natural organic matter (NOM) seriously degrade the quality of surface waters. In this study, the optimum preparation conditions of a yeast-chitosan bio-microcapsule of the Candida tropicalis strain, used to treat micro-polluted surface water, were investigated. Fourier transform
Ilias Kounatidis et al.
G3 (Bethesda, Md.), 8(5), 1637-1647 (2018-03-15)
Candida glabrata (C. glabrata) forms part of the normal human gut microbiota but can cause life-threatening invasive infections in immune-compromised individuals. C. glabrata displays high resistance to common azole antifungals, which necessitates new treatments. In this investigation, we identified five
Difco Manual, 571-571 (1998)
Thomas S Dash et al.
Structure (London, England : 1993), 27(2), 315-326 (2018-12-18)
Disulfide-rich peptides (DRPs) play diverse physiological roles and have emerged as attractive sources of pharmacological tools and drug leads. Here we describe the 3D structure of a centipede venom peptide, U-SLPTX15-Sm2a, whose family defines a unique class of one of

Articles

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

Protocoles

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

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