M8884

Methyl Green

~85% purity, powder

• Synonyme(s) : Ethyl Green
• Formule linéaire : C₂₇H₃₅BrClN₃ · ZnCl₂
• Numéro CAS: 7114-03-6
• Poids moléculaire : 653.24
• Numéro C.I. (Colour Index): 42590
• Numéro Beilstein : 3914106
• Numéro CE : 230-415-4
• Numéro MDL: MFCD00151094
• Code UNSPSC : 12171500
• ID de substance PubChem : 24897293
• Nomenclature NACRES : NA.47

Propriétés

• product name: Methyl Green, zinc chloride salt, ~85%
• Niveau de qualité: 200
• Pureté: ~85%
• Forme: powder
• Composition: Dye Content, ≥83%
• Couleur: red to very dark red
• pH: 0.2-1.8, yellow to blue
• Pf: >300 °C (lit.)
• Solubilité: water: 1 mg/mL
• εmax: >11100.00 at 417-425 nm in water at 0.005 g/L; >16000 at 311-319 nm in water at 0.005 g/L; >60000 at 626-634 nm in water at 0.005 g/L
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C
• InChI: 1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
• Clé InChI: RRKSQWOQZFIQKW-UHFFFAOYSA-J

Description

Description générale

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

Application

Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridization as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos.

Qualité

Material may be a mixture of monobromo-monochloro and dichloro ZnCl₂ salts. Halogen contents listed on label.

Remarque sur l'analyse

Dye content based on molecular weight of monobromo-monochloro ZnCl₂ salt (653.2).

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H314,H335,H411
• Conseils de prudence: P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Classification des risques: Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 8A - Combustible corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves