E1903

Ethidium homodimer I solution

≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC), 2 mM in DMSO

• Synonyme(s) : EtDi, EthD-1, Phenanthridinium, 5,5′-[1,2-ethanediylbis(imino-3,1-propanediyl)]bis(3, 8-diamino-6-phenyl)-,dichloride,dihydrochloride
• Formule empirique (notation de Hill): C₄₆H₅₀Cl₄N₈
• Poids moléculaire : 856.75
• Numéro CE : 200-664-3
• Numéro MDL: MFCD00210948
• Code UNSPSC : 12171500
• ID de substance PubChem : 24894444
• Nomenclature NACRES : NA.47

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC)
• Forme: liquid
• Concentration: 2 mM in DMSO
• Couleur: red
• ε (coefficient d'extinction): ≥9000 at 490-496 nm in water
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: −20°C
• Chaîne SMILES: Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28
• InChI: 1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
• Clé InChI: ZKAJUKABUUJRLB-UHFFFAOYSA-N

Description

Application

Ethidium homodimer I solution has been used to check membrane integrity in sperms.

Stains ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA structures.

Used to detect DNA in solution.

Actions biochimiques/physiologiques

Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity.

One molecule of EthD-1 will non-selectively bind four base pairs in dsDNA.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): 185.0 °F - closed cup
• Point d'éclair (°C): 85 °C - closed cup
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)