B9431

Biotin-4-Fluorescein

≥95% purity (HPLC), powder

• Synonyme(s) : 5-(6)-[2-(biotinoyl)aminoethylaminocarbonyl] fluorescein, B4F, Biotin (5-fluorescein) conjugate
• Formule empirique (notation de Hill): C₃₃H₃₂N₄O₈S
• Poids moléculaire : 644.69
• Code UNSPSC : 12171500
• eCl@ss : 34058011
• ID de substance PubChem : 329773319
• Nomenclature NACRES : NA.47

Propriétés

• product name: Biotin-4-Fluorescein, ≥95.0% (HPLC)
• Niveau de qualité: 100
• Pureté: ≥95.0% (HPLC)
• Forme: powder
• Poids mol.: 644.71
• Couleur: light orange to very dark orange; light yellow to very dark yellow
• ε (coefficient d'extinction): ≥70 at 491-497 nm in phosphate buffer at 0.05 M
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56

Description

Application

Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin.

Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin.

Actions biochimiques/physiologiques

Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)