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B3426

Millipore

Bile salts mixture

suitable for microbiology

Synonyme(s) :

Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture

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About This Item

Code UNSPSC :
41106212
Nomenclature NACRES :
NA.74

Stérilité

non-sterile

Niveau de qualité

100

Forme

powder

Conditionnement

pkg of 100 g

Perte

≤6% loss on drying

Couleur

yellow to brownish-yellow

pH

7±0.5

Solubilité

distilled water: freely soluble

Application(s)

microbiology

Température de stockage

10-30°C

Description générale

Bile salts mixture is a combination of an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Bile salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic, and lithocholic acids. Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that inhibits Gram-positive bacteria and induced germination. It also inhibits the outgrowth of the germinated spores but does not interfere with the growth of Enterobacteriaceae.

Application

Bile salts mixture is recommended in diverse media like MacConkey Agar, SS Agar, and Violet Red Bile Agar. It increases the selectivity of the media to Enterobacteriaceae by inhibiting Gram-positive bacteria. It is used as a selective agent in culture media for the selective growth of intestinal bacteria like E. coli, other fecal coliforms, and fecal Streptococci. But it is also used as a selective agent in media for the detection of Yersinia enterocolitica, Aeromonas hydrophila, and Vibrio species.

Notes préparatoires

Extracted from purified, fresh bile.

Mentions de danger

H412

Conseils de prudence

P273 - P501

Classification des risques

Aquatic Chronic 3

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
MKCP0424
MKCN2287
MKCC3367
MKBX4346V
MKBZ0184V

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Chhatra B Kunwar et al.
Microbiology (Reading, England), 166(12), 1149-1159 (2020-11-19)
Colonization of the gastrointestinal tract by enterohaemorrhagic Escherichia coli (EHEC) is critically dependent on its ability to sense and respond to various microenvironments within the host. EHEC exposure to physiologically relevant levels of bile salts upregulates the two-component system, pmrAB

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