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94635

Sigma-Aldrich

Gram′s safranin solution

for microscopy

Synonyme(s) :

Safranin T solution

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About This Item

Formule empirique (notation de Hill):
C20H19ClN4
Numéro CAS:
Poids moléculaire :
350.84
Numéro Beilstein :
3924099
Numéro MDL:
Code UNSPSC :
12171500
ID de substance PubChem :
Nomenclature NACRES :
MA.02

Qualité

for microscopy

Niveau de qualité

Forme

liquid

Durée de conservation

limited shelf life, expiry date on the label

Composition

ethanol, 10%

Technique(s)

microbe id | staining: suitable

Couleur

red to very dark red

Indice de réfraction

n20/D 1.339

Solubilité

water: soluble at 20 °C

Densité

0.986 g/mL at 20 °C

εmax

4.0 at 532 nm in 50% ethanol

Spectre d'activité de l'antibiotique

Gram-negative bacteria
Gram-positive bacteria

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

Clé InChI

OARRHUQTFTUEOS-UHFFFAOYSA-N

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Description générale

Gram’s safranin solution, also known as Safranin T or Basic Red 2, is a ready-to-use staining solution that comprises 10% safranin in ethanol. Safranin is a basic, cationic, azine dye containing a planar, diaminophenazine chromophore, with a phenyl substituent attached to a ring nitrogen. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. It is most notably used in bacteriology as a counterstain in Gram staining to differentiate between Gram-positive and Gram-negative bacteria.

Application

Gram’s safranin solution has been used in the following applications:
  • as a non-toxic replacement for crystal violet for the quantification of biofilm formation
  • to develop a hyaluronic acid visualization method
  • to study improved β-lactam susceptibility against biofilm-embedded staphylococcus aureus by 2-aminothiazole

Caractéristiques et avantages

  • Ready-to-use solution.
  • Modified and designed in such a way that staining can be carried out in staining cells, on the staining rack, and in automated staining systems.

Principe

Gram staining enables the differentiation of bacteria into Gram-positive and Gram-negative. The mureine structure of the bacterial cell wall is the basis of the color affinity. Bacteria are first stained with crystal violet, and then with iodine solution, which forms a dye-iodine complex. This complex is retained in the multi-layer mureine structure of the cell wall of Gram-positive bacteria after decolorization, imparting a blue-violet color. The cell wall of Gram-negative bacteria comprises a single-layered murein structure that does not retain the dye-iodine complex, allowing the uptake of safranine counterstain, and imparting a pink-red color.

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Flam. Liq. 3

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

114.8 °F

Point d'éclair (°C)

46 °C

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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