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93595

Sigma-Aldrich

Bleu de trypan solution

0.4%, for microscopy

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About This Item

Formule empirique (notation de Hill):
C34H24N6Na4O14S4
Numéro CAS:
72-57-1
Poids moléculaire :
960.81
Numéro Beilstein :
4360496
Numéro MDL:
MFCD00003969
Code UNSPSC :
12171500
ID de substance PubChem :
329770254
Nomenclature NACRES :
MA.02

Qualité

for microscopy

Niveau de qualité

200

Forme

liquid

Durée de conservation

limited shelf life, expiry date on the label

Concentration

0.4%

Technique(s)

microbe id | staining: suitable

Couleur

blue to very dark blue

Densité

1.007 g/mL at 20 °C

εmax

1.4 at 603 nm in methanol

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

Clé InChI

GLNADSQYFUSGOU-UHFFFAOYSA-J

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Catégories apparentées

Description générale

Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

Application

Trypan blue applications include:
  • it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.
  • employed as a collagen stain in a Van Gieson procedure
  • To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
  • used as a fluorescent tracer of cell populations in embryology
  • used as a tumor promoter modulating permeability of lysosomal membranes
Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.

Actions biochimiques/physiologiques

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

Forme physique

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H350

Classification des risques

Carc. 1B

Code de la classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificats d'analyse (COA)

Lot/Batch Number
BCCK2372
BCCJ9155
BCCJ0146
BCCH2945
BCCG5341

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Consulter la Bibliothèque de documents

Sheetal A Thakur et al.
Toxicological sciences : an official journal of the Society of Toxicology, 107(1), 238-246 (2008-10-07)
Alveolar macrophages (AM) in the lung have been documented to play pivotal roles in inflammation and fibrosis (silicosis) following inhalation of crystalline silica (CSiO(2)). In contrast, exposure to either titanium dioxide (TiO(2)) or amorphous silica (ASiO(2)) is considered relatively benign.
Ulrich Putz et al.
Science signaling, 5(243), ra70-ra70 (2012-09-27)
Exosomes are microvesicles of endosomal origin that are secreted, and their contents (proteins, lipids, DNA, or microRNAs) can alter the physiological states of recipient cells. We demonstrated that phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor
Georgia E Hodes et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(45), 16136-16141 (2014-10-22)
Depression and anxiety disorders are associated with increased release of peripheral cytokines; however, their functional relevance remains unknown. Using a social stress model in mice, we find preexisting individual differences in the sensitivity of the peripheral immune system that predict
Marco Todesco et al.
PLoS genetics, 10(7), e1004459-e1004459 (2014-07-11)
A fundamental question in biology is how multicellular organisms distinguish self and non-self. The ability to make this distinction allows animals and plants to detect and respond to pathogens without triggering immune reactions directed against their own cells. In plants
Kazuhiro Tanaka et al.
The Journal of clinical investigation, 125(4), 1591-1602 (2015-03-24)
The mechanistic target of rapamycin (mTOR) is hyperactivated in many types of cancer, rendering it a compelling drug target; however, the impact of mTOR inhibition on metabolic reprogramming in cancer is incompletely understood. Here, by integrating metabolic and functional studies
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