67309

Kovac′s reagent for indoles

suitable for microbiology

• Synonyme(s) : 4-(Dimethylamino)benzaldehyde solution
• Formule empirique (notation de Hill): C₉H₁₁NO
• Numéro CAS: 100-10-7
• Poids moléculaire : 149.19
• Numéro Beilstein : 4132845
• Numéro MDL: MFCD00003381
• Code UNSPSC : 41171621
• ID de substance PubChem : 329760541
• Nomenclature NACRES : NA.85

Propriétés

• Agence: according to ISO 16654:2001
• Niveau de qualité: 200
• Gamme de produits: BioChemika
• Durée de conservation: limited shelf life, expiry date on the label
• Composition: 4-(dimethlyamino)benzaldehyde, 50 g/L ; hydrochloric acid, 240 g/L ; isoamylic alcohol, 710 g/L
• Technique(s): microbe id | specific enzyme detection: suitable
• Application(s): agriculture; clinical testing; environmental; food and beverages; microbiology
• Température de stockage: 2-8°C
• Adéquation: Escherichia coli; coliforms
• Chaîne SMILES: [H]C(=O)c1ccc(cc1)N(C)C
• InChI: 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
• Clé InChI: BGNGWHSBYQYVRX-UHFFFAOYSA-N

Description

Description générale

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Application

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.

Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.

Informations sur la sécurité

• Pictogrammes: GHS02,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H226,H290,H314,H317,H332,H335
• Conseils de prudence: P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 3 - Flammable liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 109.4 °F
• Point d'éclair (°C): 43 °C
• Équipement de protection individuelle: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter