49940

ONPG Disks

suitable for microbiology

• Synonyme(s) : β-Galactosidase Test Disks, 2-Nitrophenyl-β-D-galactopyranoside Disks
• Code UNSPSC : 41171621
• Nomenclature NACRES : NA.85

Propriétés

• Agence: according to GB 4789.4-2016
• Gamme de produits: BioChemika
• Durée de conservation: limited shelf life, expiry date on the label
• Technique(s): microbe id | specific enzyme detection: suitable
• Application(s): environmental; food and beverages; microbiology
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Adéquation: Escherichia coli; bacteria

Description

Description générale

ONPG Disks are used to detect the presence of β-galactosidase, an enzyme found in lactose-fermenting organisms. Lactose utilization depends upon two enzymes: β-galactoside permease, which catalyzes transport of lactose into the cell, and β-galactosidase, which breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides including the ONPG (o-nitrophenyl-β-D-galactopyranose) substrate. ONPG hydrolysis results in the release of galactose, and the yellow chromogenic compound, o-nitrophenol. The test substrate, ONPG, does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period even for late lactose fermenters.

Conditionnement

Sterile filter paper discs (50 pcs, diameter 6 mm) impregnated with o-nitrophenyl-β-D-galactopyranose

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)