06105

Bile Esculin Azide Agar

suitable for microbiology, NutriSelect^® Plus

• Synonyme(s) : Enterococcus Selective Agar
• Code UNSPSC : 41171606
• Nomenclature NACRES : NA.85

Propriétés

• Stérilité: non-sterile
• Niveau de qualité: 200
• Forme: powder
• Durée de conservation: limited shelf life, expiry date on the label
• Composition: agar, 15.0 g/L ; beef extract, 5.0 g/L ; casein enzymic hydrolysate, 17.0 g/L ; esculin, 1.0 g/L ; ferric ammonium citrate, 0.5 g/L ; oxgall, 10.0 g/L ; proteose peptone, 3.0 g/L ; sodium azide, 0.15 g/L ; sodium chloride, 5.0 g/L
• Fabricant/nom de marque: NutriSelect^® Plus
• Technique(s): microbe id | specific enzyme detection: suitable
• Application(s): clinical testing; environmental; food and beverages; water monitoring; microbiology
• Adéquation: selective and differential for Enterococcus spp.; selective and differential for Streptococcus spp.

Description

Description générale

Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism′s potential to hydrolyze esculin. BEA comprises of oxygall (bile salts, first selective ingredients) and azide (second selective ingredients), former inhibits the growth of gram positive organisms and latter inhibits the growth of gram negative organisms. BEA also comprises of nutrients ferric citrate and esculin, where esculin is a differential ingredient and is also a fluorescent compound, upon hydrolyzation the fluorescence is lost. BEA is used for bile esculin test which is based on the hydrolysis of esculin into esculetin (6, 7-dihydroxy-coumarin) and glucose by a micro-organism that produce an enzyme esculinase.

Application

Bile Esculin Azide Agar is a selective medium used for isolation and presumptive identification of faecal Streptococci.

Notes préparatoires

Suspend 56.65g in 1 l distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes.

Note de bas de page

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Mentions de danger: H412
• Conseils de prudence: P273 - P501
• Classification des risques: Aquatic Chronic 3
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves